Protein Inhibitors - U0126-EtOH (CAS: 1173097-76-1)

U0126-EtOH (CAS: 1173097-76-1)

Catalog #：EBC51041

Biological Activity

Synonyms

U-0126 Ethanol, U0126 Ethanol

Chemical Name

(2Z,3Z)-2,3-bis[amino-(2-aminophenyl)sulfanylmethylidene]butanedinitrile;ethanol

Application

U0126-EtOH is a non-ATP competitive specific inhibitor of MEK1/2

CAS Number

1173097-76-1

Purity

≥99.0%

Molecular Weight

426.56

Molecular Formula

C₂₀H₂₂N₆OS₂

SMILES

NC1=CC=CC=C1S/C(N)=C(/C(C#N)=C(SC2=C(C=CC=C2)N)\N)C#N.CCO

Target & IC50

MEK1: IC50 = 70 nM
MEK2: IC50 = 60 nM

Shipping

Gel Pack

Storage

Store at -20°C

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	2.6282 ml	13.1409 ml	26.2819 ml
5 mM	0.5256 ml	2.6282 ml	5.2564 ml
10 mM	0.2628 ml	1.3141 ml	2.6282 ml
50 mM	0.0526 ml	0.2628 ml	0.5256 ml

Solubility
DMSO: 79 mg/mL (185.2 mM)

Product Description

U0126 was screened out as an anti-inflammatory agent that inhibited AP-1 transcription with IC50 value of 1μM and had no interactions with GREs. U0126 binds MEK1/2 in a unique site. This inhibition of MEK1/2 is noncompetitive with ERK and ATP. U0126 showed no effects on other MAPKKs. In HT22 cells, U0126 treatment significantly inhibited the cell injury caused by oxidative glutamate toxicity and remarkably blocked the phosphorylation of ERK1/2. Besides that, U0126 exerted no neuroprotection against other stimuli such as TNFα and actinomycin D. U0126 treatment also protected the primary cultured cortical neurons from oxidative glutamate toxicity and hypoxia/reoxygenation.