Stains, Dyes and Fluorescent Reagents - 2-NBDG (CAS: 186689-07-6)

2-NBDG (CAS: 186689-07-6)

Catalog #：EBC51050

Biological Activity

Synonyms

NBD-Glucose, 2-NBD-Glucose

Chemical Name

(3R,4R,5S,6R)-6-(hydroxymethyl)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)tetrahydro-2H-pyran-2,4,5-triol

Application

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability

CAS Number

C12H14N4O8

Purity

≥98.0%

Molecular Weight

342.3

Molecular Formula

C₁₂H₁₄N₄O₈

SMILES

O=C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)[C@H]([C@@H]([C@@H](CO)O)O)O

Shipping

Gel Pack

Storage

Store at -20°C

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	2.9218 ml	14.6088 ml	29.2176 ml
5 mM	0.5844 ml	2.9218 ml	5.8435 ml
10 mM	0.2922 ml	1.4609 ml	2.9218 ml
50 mM	0.0584 ml	0.2922 ml	0.5844 ml

Solubility
H2O: 5 mg/mL (14.61 mM)

Product Description

2-NBDG is a fluorescence-labeled 2-deoxy-glucose analog useful as a tracer for evaluation of cellular glucose metabolism.2-NBDG can be used in many kinds of cells in vitro, such as HepG2 human hepatocarcinoma cells, L6 rat skeletal muscle cells, MCF-7 breast cancer epithelial cells and astrocytes, it is also used in disease models, epilepsy rat, hyperglycemia, diabetes or mouse xenograft model of cancer.2-NBDG enters cells through glucose transporters and is subsequently phosphorylated by hexokinase and trapped inside cells. Flow cytometric detection of fluorescence produced by cells can be performed to examine 2-NBDG uptake into living cells, and the intracellular concentration of transported 2-NBDG can be measured with a fluorescence microplate assay. It can be detected with a fluorescence imaging microscopy or CCD camera simply as well.