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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 85 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-IKKα (Thr23) Rabbit Polyclonal Antibody detects endogenous levels of IKKα protein only when phosphorylated at Thr23. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IKKα also known as CHUK is a member of the serine/threonine protein kinase family. IKKα plays a pivotal role in activating the transcription factor NFκB, which regulates immune responses and inflammation. Located primarily in the cytoplasm, IKKα phosphorylates the inhibitory protein IκB on specific serine residues, leading to IκB degradation and subsequent NFκB release into the nucleus. This process enables NFκB to translocate and activate genes involved in immune and inflammatory responses. NFκB-inducing kinase (NIK) stimulates IκB phosphorylation by IKKα, serving as a key regulator in signaling pathways activated by tumor necrosis factor (TNF) and interleukin-1 (IL-1). The functional IKK complex, comprising IKKα, IKKβ, and IKKγ (NEMO), is essential for IκB phosphorylation, highlighting IKKα′s role in maintaining cellular homeostasis and responding to inflammatory stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on MDA-MB-435 cells treated with EGF, using Phospho-IKKα (Thr23) Rabbit Polyclonal Antibody (EAB10124) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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