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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 66 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Estrogen receptor α/ERα Rabbit Polyclonal Antibody detects endogenous levels of Estrogen receptor α/ERα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ERα(Estrogen Receptor α) also known as ESR1 is a ligand-activated transcription factor belonging to the steroid/thyroid hormone receptor superfamily. ERα plays a critical role in regulating the normal function of reproductive tissues. Post-translational modifications (PTMs) of ERα, such as phosphorylation, ubiquitination, sumoylation, acetylation, and methylation, are crucial for modulating activity, stability, cellular localization, and interactions with co-regulatory proteins. For instance, phosphorylation at specific serine residues can enhance or repress ERα′s transcriptional activity, influencing gene expression involved in cell proliferation and differentiation. Ubiquitination targets ERα for proteasomal degradation, thereby controlling receptor levels within the cell and affecting hormonal responsiveness. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, MCF7, NIH/3T3 cells, using Estrogen receptor α/ERα Rabbit Polyclonal Antibody (EAB10241) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using Estrogen receptor α/ERα Rabbit Polyclonal Antibody (EAB10241) at 1:100 dilution.
 Immunofluorescence analysis of A549 cell, using Estrogen receptor α/ERα Rabbit Polyclonal Antibody (EAB10241) at 1:200 dilution.
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