www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 50 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:100-5000 IHC-P=1:50-300 IF/ICC=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GFAP Mouse Monoclonal Antibody detects endogenous levels of GFAP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GFAP(Glial fibrillary acidic protein) is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino-terminal head domain, a central rod domain and a carboxy-terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A431, PC12, Mouse brain, Rat brain cells, using GFAP Mouse Monoclonal Antibody (EAB10257) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 50 kDa; Observed band size: 45 kDa  Immunohistochemical analysis of paraffin-embedded Rat brain tissue, using GFAP Mouse Monoclonal Antibody (EAB10257) at 1:500 dilution.
 Immunofluorescence analysis of Mouse brain tissue, using GFAP Mouse Monoclonal Antibody(EAB10257)(red) at 1:200 dilution.
|
