| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 27 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | VEGF-A Rabbit Polyclonal Antibody detects endogenous levels of VEGF-A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
VEGF-A also known as VPF, VEGF is a member of the PDGF/VEGF growth factor family. VEGF-A plays a crucial role in angiogenesis, the process of new blood vessel formation, which is vital for supplying nutrients and oxygen to tissues, particularly during development and in response to injury. VEGF-A dysregulation is implicated in various pathological conditions, including cancer, where VEGF-A promotes tumor growth and metastasis by enhancing blood supply to the tumor microenvironment. VEGF-A exists as a dimeric glycoprotein and exhibits structural similarities to platelet-derived growth factor (PDGF), with multiple isoforms generated through alternative mRNA splicing. VEGF-A expression is tightly regulated, and VEGF-A mRNA is often found in primary tumors, indicating VEGF′s potential as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc, MDA-MB-231, MKN45 cells, using VEGF-A Rabbit Polyclonal Antibody (EAB10292) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue, using VEGF-A Rabbit Polyclonal Antibody (EAB10292) at 1:200 dilution.
 Immunofluorescence analysis of Mouse kidney tissue, using VEGF-A Rabbit Polyclonal Antibody (EAB10292)(red) at 1:200 dilution(4°C overnight).
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