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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 58 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-ICAM-1/CD54 (Tyr512) Rabbit Polyclonal Antibody detects endogenous levels of ICAM-1/CD54 protein only when phosphorylated at Tyr512. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ICAM-1 also known as CD54 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. ICAM-1 plays a crucial role in immune responses by mediating leukocyte adhesion and transmigration across the endothelium during inflammation. ICAM-1 undergoes post-translational modifications such as N-linked glycosylation, which are important for stability, cell surface expression, and interaction with integrins like LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) on leukocytes. These modifications can affect ICAM-1′s function in cell adhesion and signaling pathways. Altered expression or glycosylation patterns of ICAM-1 are associated with various pathological conditions, including autoimmune diseases, cardiovascular disorders, and cancer metastasis. Understanding ICAM-1′s role and post-translational modifications is essential for research into inflammatory processes and potential therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with TNFalpha , using Phospho-ICAM-1/CD54 (Tyr512) Rabbit Polyclonal Antibody (EAB10308) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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