| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 53 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cyclin A Rabbit Polyclonal Antibody detects endogenous levels of Cyclin A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin A also known as Cyclin A1/A2 belongs to the highly conserved cyclin family. Cyclin A plays a pivotal role in the regulation of the eukaryotic cell cycle, particularly during the transition from the S phase to mitosis, making cyclin A essential for proper cell division and proliferation. Cyclin A is primarily located in the nucleus, where cyclin A associates with cyclin-dependent kinases, particularly Cdk2, to facilitate the progression of the cell cycle. Nuclear localization of cyclin A is crucial as cyclin A allows for the timely activation of target proteins necessary for DNA replication and cell cycle progression. Furthermore, cyclin A has been shown to interact with various proteins, including the retinoblastoma protein and transcription factors such as E2F, which are vital for regulating gene expression during the cell cycle. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Jurkat, NIH/3T3 cells, using Cyclin A Rabbit Polyclonal Antibody (EAB11013) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using Cyclin A Rabbit Polyclonal Antibody (EAB11013) at 1:100 dilution. The image on the right is blocked with the synthesized peptide.
 Immunofluorescence nalysis of Hela cell, using Cyclin A Rabbit Polyclonal Antibody (EAB10368)(red) at 1:200 dilution(4°C overnight).
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