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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 38 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Cyclin H (Thr315) Rabbit Polyclonal Antibody detects endogenous levels of Cyclin H protein only when phosphorylated at Thr315. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin H also known as CCNH is a member of the highly conserved cyclin family, Cyclin H plays a crucial role in cell cycle regulation as a component of the cyclin-dependent kinase-activating kinase (CAK) complex, which is essential for the activation of cyclin-dependent kinases (Cdks) that drive cell cycle progression. Cyclin H serves as the regulatory subunit of the CAK, which also includes the catalytic subunit Cdk7. Activation of Cdks, such as Cdk2, is vital for the transition through different phases of the cell cycle, and cyclin H′s interaction with Cdk7 is necessary for the phosphorylation of key threonine residues that enable full enzymatic activity. This phosphorylation is critical, as cyclin H can effectively regulate cell cycle checkpoints, thereby maintaining proper cellular function and preventing uncontrolled cell division, which is a hallmark of cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using Phospho-Cyclin H (Thr315) Rabbit Polyclonal Antibody (EAB10387) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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