| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 26 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bcl-2 Rabbit Polyclonal Antibody detects endogenous levels of Bcl-2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Bcl-2 plays a crucial role as a key regulator of apoptosis, which is vital for normal development, maintaining tissue homeostasis, and providing defense against pathogens. Immunostaining of human tissues reveals that Bcl-2 is primarily localized in the cytoplasm and associated with the membrane, highlighting its function as an anti-apoptotic oncoprotein. Bcl-2 promotes cell survival by engaging in protein-protein interactions with other members of the Bcl-2 family, including the anti-apoptotic proteins Bcl-xL, Mcl-1, Bcl-w, and A1, as well as the pro-apoptotic proteins Bax, Bak, Bik, Bad, and Bid. Regulation of Bcl-2′s anti-apoptotic function can occur through mechanisms such as proteolytic processing and phosphorylation, which are essential for its activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, NIH/3T3, K562 cells, using Bcl-2 Rabbit Polyclonal Antibody (EAB10404) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue, using Bcl-2 Rabbit Polyclonal Antibody (EAB10404) at 1:100 dilution.
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