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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 49 | Human, Monkey |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg4C Rabbit Polyclonal Antibody detects endogenous levels of Atg4C protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg4C is a member of the C-54 family of cysteine proteases. Atg4C plays a crucial role in autophagy process, essential for cellular homeostasis and degradation of damaged organelles and proteins. Atg4C is predominantly expressed in skeletal muscle, heart, liver, and testis, where Atg4C localizes to cytoplasm. As a cysteine protease, Atg4C is responsible for cleaving C-terminal region of proteins such as MAP1LC3, GABARAPL2, or GABARAP, thereby facilitating formation of autophagosomes. Regulation of Atg4C activity is also significant, as N-ethylmaleimide can inhibit Atg4C, highlighting Atg4C′s importance in autophagy pathway and potential implications in cancer and neurodegenerative diseases. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, COS7 cells, using Atg4C Rabbit Polyclonal Antibody (EAB10493) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue, using Atg4C Rabbit Polyclonal Antibody (EAB10493) at 1:100 dilution.
 Immunofluorescence analysis of HucEc cell, using Atg4C Rabbit Polyclonal Antibody (EAB10493) at 1:100 dilution.
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