| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB | 52 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Beclin-1 Rabbit Polyclonal Antibody detects endogenous levels of Beclin-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Beclin-1 also known as BECN1, ATG6, VPS30, Beclin1 is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. Beclin 1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the Ribosomal Protein S6, and both are mediated via amino acids. Beclin 1 expression in various carcinoma cell lines such as MCF7 is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, Beclin 1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, Beclin 1 shares structural similarity to the yeast autophagy gene product, Apg6, and was one of the first mammalian proteins discovered to mediate autophagy. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T, Jurkat, C2C12, Rat Kidney cells, using Beclin-1 Rabbit Polyclonal Antibody (EAB10496) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 52 kDa; Observed band size: 60 kDa |
