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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 28 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-14-3-3 beta/zeta (Ser184/186) Rabbit Polyclonal Antibody detects endogenous levels of 14-3-3 beta/zeta only when phosphorylated at Ser184/186. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 beta/zeta is a protein belonging to the 14-3-3 family of proteins.14-3-3 beta/zeta protein plays a crucial role in regulating numerous cellular processes, particularly in cancer biology, where 14-3-3 beta/zeta protein is involved in apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 beta/zeta protein is primarily located in the cytoplasm, where 14-3-3 beta/zeta protein acts as a scaffold that facilitates the interaction of various signaling proteins, thereby influencing their activity and stability. This cytoplasmic localization allows 14-3-3 proteins to sequester target proteins, preventing their degradation and modulating their functions in response to cellular signals. 14-3-3 proteins bind to phosphorylated serine and threonine residues, enabling regulation of a wide array of cellular pathways, and dysregulation can lead to significant pathological conditions, including cancer. |
| Product Image Gallery | |
 Western blot analysis of lysates on RAW264.7 cells treated with UV, using Phospho-14-3-3 beta/zeta (Ser184/186) Rabbit Polyclonal Antibody (EAB10502) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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