| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 28 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 14-3-3 gamma Rabbit Polyclonal Antibody detects endogenous levels of 14-3-3 gamma protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 gamma is a member of the 14-3-3 protein family, which plays a crucial role in regulating various cellular processes, including apoptosis, mitogenic signaling, and cell-cycle checkpoints, all vital for maintaining cellular homeostasis and preventing cancer progression. 14-3-3 gamma is primarily located in the cytoplasm, where 14-3-3 gamma acts as a scaffolding protein that facilitates interaction between different signaling molecules. Cytoplasmic localization of 14-3-3 gamma allows for rapid modulation of signaling pathways in response to cellular stimuli, ensuring appropriate cellular responses are activated or inhibited as needed. By binding to phosphorylated serine and threonine residues on target proteins, 14-3-3 gamma can influence stability, localization, and activity, thereby playing a pivotal role in regulating key cellular functions. Formation of dimers and interaction with various ligands by 14-3-3 gamma underscores importance in cellular signaling networks, and any loss of expression or dysfunction can lead to a range of pathological conditions, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using 14-3-3 gamma Rabbit Polyclonal Antibody (EAB10503) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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