| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 28 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 14-3-3 pan Rabbit Polyclonal Antibody detects endogenous levels of total 14-3-3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 proteins play a crucial role in regulating numerous cellular processes, particularly those associated with cancer biology, such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 proteins are located primarily in the cytoplasm and nucleus, where 14-3-3 proteins interact with a wide range of target proteins, influencing their activity and stability. 14-3-3 proteins′ ability to sequester ligands in the cytoplasm is particularly important, as this sequestration prevents ligand degradation and modulates interactions with other cellular components. Such dynamic regulation is essential for maintaining cellular homeostasis and ensuring proper cellular responses to various stimuli. Loss of 14-3-3 expression can lead to dysregulation of these processes, contributing to the development of various diseases, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T cells, using 14-3-3 pan Rabbit Polyclonal Antibody (EAB11874) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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