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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF, ELISA | 72 | Human, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:50-300 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GRP78/BiP Mouse Monoclonal Antibody detects endogenous levels of GRP78/BiP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GRP78(Glucose regulated protein 78) also known as BiP, HSPA5 is a member of the heat shock protein 70 (HSP70) family, primarily localized in the endoplasmic reticulum (ER) of cells. GRP78′s presence in the ER is vital as a molecular chaperone, ensuring correct folding and assembly of nascent proteins while maintaining protein homeostasis. This function is essential for preventing misfolded protein accumulation, which can lead to ER stress and activate the unfolded protein response (UPR), a critical cellular stress response associated with various diseases, including cancer and neurodegenerative disorders. GRP78 interacts with several specific proteins, including ATF6, IRE1, and PERK, which are key players in the UPR pathway. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Rat liver cells, using GRP78/BiP Mouse Monoclonal Antibody (EAB10513) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 72 kDa; Observed band size: 78 kDa  Immunohistochemical analysis of paraffin-embedded Human colon caricnoma tissue, using GRP78/BiP Mouse Monoclonal Antibody (EAB10513) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast caricnoma tissue, using GRP78/BiP Mouse Monoclonal Antibody (EAB10513) at 1:200 dilution.
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