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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 72 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Wee1 (Ser642) Rabbit Polyclonal Antibody detects endogenous levels of wee1 protein only when phosphorylated at Ser642. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Wee1 is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. Wee 1 protein plays a critical role in cell cycle regulation by acting as a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15, which is essential for maintaining Cdc2 in an inactive state during the S and G2 phases of the cell cycle. This regulation prevents premature entry into mitosis, thereby ensuring proper cell division and genomic stability. Additionally, Wee 1 activity is tightly controlled throughout the cell cycle, with function being complemented by Myt 1, another member of the Wee 1 family that phosphorylates Cdc2 on both threonine 14 and tyrosine 15. The interplay between these kinases and the phosphatase Cdc25, which dephosphorylates Cdc2 to activate the Cdc2/cyclin B complex, highlights the intricate balance of phosphorylation and dephosphorylation that governs cell cycle progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293 cells treated with Etoposide, using Phospho-Wee1 (Ser642) Rabbit Polyclonal Antibody (EAB10561) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 72 kDa; Observed band size: 100 kDa |
