| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 50 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BLNK Rabbit Polyclonal Antibody detects endogenous levels of BLNK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BLNK aslo known as SLP65 is a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. BLNK is essential for B cell activation, proliferation, and differentiation. Located primarily in the cytoplasm, BLNK acts as a central hub for signaling pathways initiated by BCR engagement, facilitating the recruitment and activation of downstream effectors such as GRB2, Vav, Nck, and phospholipase C gamma (PLC γ). BLNK′s location in the cell enables signal integration from various kinases, including Syk and Lyn, which are vital for proper B cell development and function. BLNK exists in two isoforms, pp68 and pp70, resulting from alternative splicing of the human BLNK gene transcript, and phosphorylation by Syk is a key regulatory step that enables the activation of downstream signaling cascades. |
| Product Image Gallery | |
 Western blot analysis of extracts on LOVO, HuvEc cells, using BLNK Rabbit Polyclonal Antibody (EAB10648) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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