| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 175 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-eIF4G (Ser1108) Rabbit Polyclonal Antibody detects endogenous levels of eIF4G only when phosphorylated at Ser1108. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
eIF4G also known as P220, eIF4G1 is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis.eIF4G plays a crucial role in the initiation of protein synthesis in eukaryotic cells, functioning as a key component of the eukaryotic initiation factor 4F (eIF4F) complex, which is essential for the recruitment of ribosomes to messenger RNA (mRNA). This complex is vital for the translation process, as eIF4G serves as a scaffold that links eIF4E, which binds to the mRNA cap, and eIF4A, an ATP-dependent RNA helicase that unwinds mRNA secondary structures. By facilitating these interactions, eIF4G ensures efficient translation initiation, which is critical for proper cellular function and response to environmental signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cell treated with Heat-shock, using Phospho-eIF4G (Ser1108) Rabbit Polyclonal Antibody (EAB10731) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 175 kDa; Observed band size: 220 kDa |
