www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 109 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GKAP Rabbit Polyclonal Antibody detects endogenous levels of GKAP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GKAP(Guanylate kinase associated protein) also known as DLGAP1, SAPAP1 is part of the postsynaptic scaffolding complex that includes the PSD-95, SAP90, and SHANK proteins. GKAP links the synaptic protein SHANK to a PSD-95 complex that includes NMDA glutamate receptors. Synaptic activity induces ubiquitination of GKAP protein by the E3 ubiquitin ligase TRIM3, which results in decreased GKAP protein levels through degradation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, 293 cells, using GKAP Rabbit Polyclonal Antibody (EAB10774) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using GKAP Rabbit Polyclonal Antibody (EAB10774) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using GKAP Rabbit Polyclonal Antibody (EAB10774) at 1:100 dilution.
|
