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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 60 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-HCK (Tyr521) Rabbit Polyclonal Antibody detects endogenous levels of HCK protein only when phosphorylated on Tyr521. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HCK(Hematopoietic cell kinase) is a member of the Src family of tyrosine kinases. HCK plays a crucial role in hematopoietic signaling pathways, particularly in the regulation of immune cell functions. HCK is predominantly expressed in hematopoietic cells, especially granulocytes, where Hck is involved in processes such as cell proliferation, differentiation, and migration. HCK′s importance lies in its ability to modulate signaling cascades that govern immune responses, making HCK a vital player in the body′s defense mechanisms. HCK is located on chromosome 20q11-q12 and encodes a 505 amino acid protein, which is typically maintained in an inactive state and can be activated during cellular events like mitosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Phospho-HCK (Tyr521) Rabbit Polyclonal Antibody (EAB10833) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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