| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 57 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PNK1 Rabbit Polyclonal Antibody detects endogenous levels of PNK1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PNK1 also known as PNK, PNKP is a member of the DNA 3′ phosphatase family. PNK1 plays a crucial role in DNA repair mechanisms, particularly in response to damage from radiation or oxidative stress. PNK1 encoded by the PNKP gene, features a 3′-phosphatase domain that shares similarities with L-2-haloacid dehalogenases and possesses an ATP binding site, which is essential for enzymatic activity. PNK1 is predominantly located in the nucleus, where PNK1 catalyzes the phosphorylation of DNA at hydroxy termini while also having the ability to dephosphorylate 3′-phosphate termini. PNK1 expression is notably high in tissues such as the testis, pancreas, spleen, kidney, and heart, highlighting its importance in cellular processes related to DNA integrity and repair. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cell, using PNK1 Rabbit Polyclonal Antibody (EAB10928) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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