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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 51 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Mnk1 (Thr385) Rabbit Polyclonal Antibody detects endogenous levels of Mnk1 only when phosphorylated at Thr385. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Mnk1 also known as MKNK1 is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may plays a crucial role in cellular signaling pathways, particularly in regulating protein synthesis in response to stress signals. Mnk1 is primarily located in the cytoplasm, where Mnk1 interacts with eukaryotic translation initiation factor 4E (eIF4E), a key player in translation initiation. This interaction enables eIF4E phosphorylation, which enhances affinity for the mRNA cap structure, promoting translation of specific mRNAs critical for cell survival and proliferation under stress conditions. Mnk1′s ability to modulate protein synthesis in response to environmental cues underscores Mnk1′s importance in various physiological processes, including cell growth, differentiation, and stress response, making Mnk1 a significant target for research in cancer and other diseases. |
| Product Image Gallery | |
 Western blot analysis of lysates on COLO205 cells treated with PMA, using Phospho-Mnk1 (Thr385) Rabbit Polyclonal Antibody (EAB11717) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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