| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 73 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-LIMK1 (Thr508) Rabbit Polyclonal Antibody detects endogenous levels of LIMK1 only when phosphorylated at Thr508. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
LIMK1(LIM Kinase 1) is a member of the LIM kinase family. LIMK1 plays a crucial role in regulating the actin cytoskeleton by phosphorylating and inactivating Cofilin, a protein that severs actin filaments. This regulation is vital for maintaining cellular structure and motility, as LIMK1 influences processes such as cell migration, morphogenesis, and neuronal differentiation. LIMK1′s ability to modulate actin dynamics is particularly important in developmental biology and cancer metastasis, where altered cell movement can lead to significant physiological changes. Expression patterns of LIMK1 and LIMK2 suggest distinct roles during development, with LIMK1 being implicated in inhibiting neuronal differentiation in certain contexts. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using Phospho-LIMK1 (Thr508) Rabbit Polyclonal Antibody (EAB11137) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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