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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 28 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-LAT (Tyr220) Rabbit Polyclonal Antibody detects endogenous levels of LAT only when phosphorylated at Tyr220. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT plays a significant role in T cell activation by serving as a signaling hub. Upon T cell receptor engagement, LAT associates with various signaling molecules, including phospholipase C gamma 1 (PLC γ1), GRB2, and the p85 subunit of phosphoinositide 3-kinase (PI 3-kinase), facilitating a cascade of intracellular signaling events that lead to T cell proliferation and differentiation. Notably, LAT undergoes palmitoylation, a post-translational modification that is essential for tyrosine phosphorylation and proper localization to glycolipid-enriched microdomains within the cell membrane. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells, using Phospho-LAT (Tyr220) Rabbit Polyclonal Antibody (EAB14564) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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