| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 12 | Human, Mouse, Rat, Chicken |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cytochrome C Mouse Monoclonal Antibody detects endogenous levels of Cytochrome C protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cytochrome c also known as somatic cytochrome c, CYCS is a small heme protein located in the mitochondrial intermembrane space. Such localization allows cytochrome c to play a key role in the electron transport chain, transferring electrons between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase), which is essential for ATP production during oxidative phosphorylation. cytochrome c′s position in the mitochondria enables participation in the intrinsic pathway of apoptosis; upon release into the cytosol, cytochrome c interacts with apoptotic protease activating factor-1 (Apaf-1) to form the apoptosome, leading to the activation of caspase-9 and downstream effector caspases like caspase-3. Release of cytochrome c from mitochondria is a pivotal event in programmed cell death, and dysregulation of this process is associated with diseases such as cancer and neurodegenerative disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, NIH/3T3, Mouse liver, Rat liver, Rat Kidney cells, using Cytochrome C Mouse Monoclonal Antibody (EAB14621) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human Breast caricnoma tissue, using Cytochrome C Mouse Monoclonal Antibody (EAB14621) at 1:500 dilution.
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