| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 36 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Acetyl-Ape1/Ref-1 (Lys6) Rabbit Polyclonal Antibody detects endogenous levels of Ape1/Ref-1 only when acetylated at Lys6. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ape1 also known as Ref-1, Redox factor 1 is a multifunctional protein with several biological activities. These include roles in DNA repair and in the cellular response to oxidative stress. Ape1 plays a crucial role in DNA repair and redox regulation of transcription factors, which is vital for maintaining cellular homeostasis and responding to oxidative stress. Located primarily in the nucleus, Ape1 is essential for the repair of apurinic/apyrimidinic sites in DNA, which can arise from oxidative damage or spontaneous hydrolysis. This repair function is critical because unrepaired DNA lesions can lead to mutations, genomic instability, and contribute to the development of various diseases, including cancer. Additionally, Ape1′s redox modulation of transcription factors enhances DNA binding activity, thereby influencing gene expression in response to cellular stress. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549 cells, using Acetyl-Ape1/Ref-1 (Lys6) Rabbit Polyclonal Antibody (EAB14635) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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