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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 37 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ICAD/DFF45 Rabbit Polyclonal Antibody detects endogenous levels of ICAD/DFF45 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ICAD also known as DFF45, DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when ICAD is cleaved by caspase-3. ICAD plays a crucial role in the apoptotic process by acting as an inhibitor of the DNA fragmentation factor, which is essential for the controlled dismantling of cellular components during apoptosis. When cleaved by caspases, particularly CPP32, ICAD releases its inhibitory effect, allowing the activation of the DNA fragmentation factor that leads to the fragmentation of nuclear DNA, a hallmark of apoptosis. This process is vital for maintaining tissue homeostasis and preventing the development of cancer, as ICAD ensures damaged or unnecessary cells are efficiently removed from the organism. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, NIH/3T3 cells, using ICAD/DFF45 Rabbit Polyclonal Antibody (EAB14650) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ICAD/DFF45 Rabbit Polyclonal Antibody (EAB14650) at 1:100 dilution.
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