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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 38 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TOB1 Rabbit Polyclonal Antibody detects endogenous levels of TOB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TOB1 also known as TROB, APRO6 is a member of the transducer of erbB-2 /B-cell translocation gene protein family. TOB1plays a crucial role in regulating cell cycle progression, particularly in the transition from G0/G1 phase to S phase, through interactions with the mammalian homologue of yeast Caf1. This regulation is vital for maintaining proper cellular proliferation and function, especially in T lymphocytes, where TOB1 can interact with Smad2/4 to enhance SMAD DNA binding to the interleukin-2 promoter, ultimately inhibiting IL-2 transcription. Additionally, TOB1′s phosphorylation at specific serine residues by ERK1/2 upon growth-factor stimulation highlights involvement in signaling pathways that influence cell growth and differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HT29 cells, using TOB1 Rabbit Polyclonal Antibody (EAB14668) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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