| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 96 | Human, Chicken |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Dnmt3b Rabbit Polyclonal Antibody detects endogenous levels of Dnmt3b protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Dnmt3b plays a crucial role in the regulation of gene expression through DNA methylation, a process vital for normal development and cellular differentiation. Specifically, Dnmt3b is responsible for de novo methylation, which establishes new methylation patterns on previously unmethylated DNA, particularly in embryonic stem cells. This function is essential for silencing genes that should not be expressed in certain cell types, thereby maintaining cellular identity and function. The ability of Dnmt3b to add methyl groups to cytosines within CpG dinucleotides is critical for proper regulation of gene expression, and dysregulation of this process can lead to various diseases, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HT29 cells, using Dnmt3b Rabbit Polyclonal Antibody (EAB14693) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma tissue, using Dnmt3b Rabbit Polyclonal Antibody (EAB14693) at 1:200 dilution.
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