| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 168 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-TRAP220/MED1 (Thr1457) Rabbit Polyclonal Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Thr1457. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TRAP220 also known as MED1 is integral to forming functional transcriptional machinery. TRAP220 is primarily located in the nucleus, where TRAP220 facilitates hormone-induced transcriptional activation by binding to nuclear receptors, including thyroid hormone receptors and vitamin D receptors. Nuclear localization of TRAP220 allows precise regulation of gene expression in response to hormonal signals, impacting various physiological processes such as metabolism, development, and cellular differentiation. TRAP220 is widely expressed in tissues such as skeletal muscle, heart, and placenta, highlighting TRAP220′s importance in both developmental and metabolic pathways. Through interactions with other components of the TRAP complex, making TRAP220 a significant target for studies related to gene regulation and endocrine signaling. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with Serum, using Phospho-TRAP220/MED1 (Thr1457) Rabbit Polyclonal Antibody (EAB14749) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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