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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 35 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CREB Rabbit Monoclonal Antibody detects endogenous levels of CREB protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CREB also known as CREB-1 is a crucial transcription factor that regulates gene expression by binding to the cAMP response element (CRE) in the promoters of target genes, thereby modulating essential cellular processes such as metabolism, neuronal plasticity, and survival. Upon phosphorylation at Serine 133 by protein kinase A, CREB-1 undergoes a conformational change that facilitates interaction with the CREB-binding protein (CBP), promoting the assembly of the basal transcription complex and activating transcriptional programs critical for cellular responses to hormonal and stress signals. This regulatory mechanism underscores CREB-1′s pivotal role in neoglucogenesis through interaction with the nuclear coactivator PGC-1, as well as involvement in the pathogenesis of type II diabetes and dilated cardiomyopathy, highlighting significance in both metabolic regulation and cardiovascular health. |
| Product Image Gallery | |
 Western blot analysis of extracts on different cells, using CREB Rabbit Monoclonal Antibody (EAB21318) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: Jurkat Lane2: NIH/3T3 Predicted band size: 35 kDa; Observed band size: 35-46 kDa |
