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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 72 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-c-Myb (Ser11) Rabbit Monoclonal Antibody detects endogenous levels of c-Myb only when phosphorylated at Ser11. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
c-Myb is a protein with three HTH DNA-binding domains that functions as a transcription regulator. c-Myb is predominantly localized in the nucleus, where c-Myb functions as a critical transcription factor regulating the expression of genes involved in hematopoietic cell proliferation, differentiation, and survival. c-Myb may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. This nuclear localization is essential for c-Myb′s role in controlling transcriptional programs that maintain the balance between cell growth and differentiation, making c-Myb a vital component in normal hematopoiesis and implicated in oncogenic processes when dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using Phospho-c-Myb (Ser11) Rabbit Monoclonal Antibody (EAB21245) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 72 kDa; Observed band size: 80 kDa |
