| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB | 62 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PKR (Thr451) Rabbit Monoclonal Antibody detects endogenous levels of PKR only when phosphorylated at Thr451. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKR also known as EIF2AK2 is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. PKR plays a crucial role in the cellular response to viral infections by acting as an interferon-inducible, RNA-dependent protein serine/threonine kinase. PKR′s primary function is to phosphorylate the α subunit of the protein synthesis initiation factor eIF-2, specifically at serine-51, which leads to the inhibition of translation and effectively halts viral replication. This mechanism is vital for the host′s defense against viral pathogens, as PKR prevents the production of viral proteins. PKR is characterized by distinct structural domains, with the serine/threonine kinase catalytic domains located in the carboxy-terminal half and the RNA-binding domains situated in the amino-terminal region. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with TNFalpha, using Phospho-PKR (Thr451) Rabbit Monoclonal Antibody (EAB22684) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 62 kDa; Observed band size: 68 kDa |
