www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 22 | Human, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:10-100 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | p27 Kip1 Rabbit Monoclonal Antibody detects endogenous levels of p27 Kip1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p27 Kip1 also known as CDKN1B is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 Kip1 plays a crucial role as a negative regulator of cell cycle progression, particularly during the G1 phase, where p27 Kip1 inhibits the activity of cyclin-dependent kinases (Cdks) by binding to D-type cyclins and Cdk4, thereby preventing the transition to the S phase. This regulation is vital for maintaining proper cell growth and division, especially in response to external signals such as transforming growth factor-beta (TGFβ), which can induce G1 arrest. The ability of p27 Kip1 to modulate cell cycle progression highlights its importance in tumor suppression, as dysregulation of p27 Kip1 can lead to uncontrolled cell proliferation and cancer development. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, PC12, Jurkat cells, using p27 Kip1 Rabbit Monoclonal Antibody (EAB21517) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 22 kDa; Observed band size: 27 kDa |
