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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 161 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | nNOS/NOS1 Rabbit Monoclonal Antibody detects endogenous levels of nNOS/NOS1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
nNOS also known as NOS1 belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. nNOS is predominantly localized in neurons, where NOS1 interacts with key proteins such as PSD-95, which anchors nNOS to the postsynaptic density, and calmodulin, which regulates enzymatic activity. These interactions are crucial for the precise spatial and temporal production of NO, facilitating NO′s role in modulating synaptic strength and plasticity. Understanding the protein-protein interactions of nNOS is vital for elucidating the mechanisms underlying neuronal signaling and the pathophysiology of neurological disorders where NO signaling may be disrupted. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse heart cells, using nNOS/NOS1 Rabbit Monoclonal Antibody (EAB21564) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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