| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | FTH1 Rabbit Monoclonal Antibody detects endogenous levels of FTH1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FTH1 also known as FTH, Ferritin heavy chain 1 plays a crucial role in iron metabolism, primarily by catalyzing the oxidation of ferrous iron (FeII) to ferric iron (FeIII), which is essential for safe iron storage within cells. This process is vital because excess free iron can generate harmful reactive oxygen species, leading to cellular damage. By facilitating the initial step of iron storage, FTH1 helps maintain cellular iron homeostasis and protects against oxidative stress. Additionally, FTH1 is implicated in the regulation of thymidine biosynthesis, enhancing the expression of cytoplasmic serine hydroxymethyltransferase, a key enzyme in the synthesis of thymidylat. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562 cells, using FTH1 Rabbit Monoclonal Antibody (EAB21654) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human kidney tissue, using FTH1 Rabbit Monoclonal Antibody (EAB21654) at 1:200 dilution.
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