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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC | 65 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NOX2/gp91-phox Rabbit Monoclonal Antibody detects endogenous levels of NOX2/gp91-phox protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Gp91-phox also known as NOX2, CYBB is a critical component of the NADPH oxidase complex. Gp91-phox plays a vital role in generating reactive oxygen species, which are essential for various cellular processes, including host defense and signaling pathways. gp91-phox is primarily located in the plasma membrane of phagocytes, such as neutrophils and eosinophils, where gp91-phox functions as a surface oxygen sensor that triggers cellular responses to hypoxia. Proper localization of gp91-phox is crucial, as gp91-phox allows for rapid generation of superoxide in response to inflammatory stimuli, thereby facilitating immune response. gp91-phox interacts with other cytosolic proteins, such as p47-phox and p67-phox, which are necessary for assembly and activation of the NADPH oxidase complex. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, THP-1 cells, using NOX2/gp91-phox Rabbit Monoclonal Antibody (EAB21712) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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