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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, FC, ELISA | 54 | Human, Mouse, Rat, Monkey |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | YAP Mouse Monoclonal Antibody detects endogenous levels of YAP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
YAP also known YAP1 is a pivotal component of the Hippo signaling pathway, functioning as a transcriptional co-activator that regulates essential cellular processes including cell proliferation, apoptosis, and organ size. The localization of YAP within the cell, whether in the nucleus or cytoplasm, is crucial for controlling gene expression and maintaining cellular homeostasis. By interacting with 14-3-3 proteins, YAP′s activity and subcellular distribution are tightly regulated, linking extracellular signals to nuclear responses that influence cell differentiation, cell cycle progression, and programmed cell death. This regulation is vital for normal physiological functions and is implicated in the development of various cancers when dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, PC12 cells, using YAP Mouse Monoclonal Antibody (EAB21329) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 54 kDa; Observed band size: 54-75 kDa  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using YAP Mouse Monoclonal Antibody (EAB21329) at 1:200 dilution.
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