| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 38 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cyclin H Rabbit Monoclonal Antibody detects endogenous levels of Cyclin H protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin H also known as CCNH is a member of the highly conserved cyclin family, Cyclin H plays a crucial role in cell cycle regulation as a component of the cyclin-dependent kinase-activating kinase (CAK) complex, which is essential for the activation of cyclin-dependent kinases (Cdks) that drive cell cycle progression. Cyclin H serves as the regulatory subunit of the CAK, which also includes the catalytic subunit Cdk7. Activation of Cdks, such as Cdk2, is vital for the transition through different phases of the cell cycle, and cyclin H′s interaction with Cdk7 is necessary for the phosphorylation of key threonine residues that enable full enzymatic activity. This phosphorylation is critical, as cyclin H can effectively regulate cell cycle checkpoints, thereby maintaining proper cellular function and preventing uncontrolled cell division, which is a hallmark of cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, K562 cells, using Cyclin H Rabbit Monoclonal Antibody (EAB21747) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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