| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC | 156 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Insulin Receptor α Rabbit Monoclonal Antibody detects endogenous levels of Insulin Receptor α protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Insulin receptor is a crucial heterotetrameric protein composed of two extracellular alpha subunits and two transmembrane beta subunits, which are connected by disulfide bonds. The alpha subunits are responsible for binding insulin, initiating conformational changes that activate the receptor′s intrinsic tyrosine kinase activity in the beta subunits. This structural configuration is vital for the receptor′s function in mediating insulin′s metabolic effects, such as facilitating glucose uptake into cells, promoting glycogen synthesis, and regulating various aspects of cellular metabolism and growth. Upon insulin binding, the receptor undergoes autophosphorylation of its tyrosine kinase domains, triggering a cascade of downstream signaling pathways that enhance glucose transport, increase cell permeability to monosaccharides, amino acids, and fatty acids, and maintain metabolic homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, 293 cells, using Insulin Receptor α Rabbit Monoclonal Antibody (EAB21234) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 156 kDa; Observed band size: 95 kDa |
