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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, ELISA | 43 | Human, Pig |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TFG Mouse Monoclonal Antibody detects endogenous levels of TFG protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TFG(TRK fused gene) also known as TRK-T3 is a fusion protein resulting from oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, and is particularly significant in thyroid carcinomas where these rearrangements are frequently observed. TFG protein is characterized by a coiled-coil motif within the TFG region, which is crucial for forming protein complexes, thereby facilitating various signaling pathways. This structural feature allows TFG to interact with key adaptor proteins such as Shc and SNT1/FRS2, which play essential roles in linking receptor tyrosine kinases to the mitogen-activated protein kinase pathway, ultimately influencing cellular proliferation and survival. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, MCF, PC3 cells, using TFG Mouse Monoclonal Antibody (EAB21769) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 43 kDa; Observed band size: 50-55 kDa |
