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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 75 | Human, Mouse, Rat, Pig |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:500-2000 FC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ATF-6 Mouse Monoclonal Antibody detects endogenous levels of ATF-6 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ATF-6(Activating transcription factor 6) also known ATF6A is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. ATF-6 functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. ATF-6 protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in ATF-6 with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. ATF-6 is also thought to be a potential therapeutic target for cystic fibrosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, K562, MCF7 cells, using ATF-6 Mouse Monoclonal Antibody (EAB21835) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 75 kDa; Observed band size: 90-100 kDa |
