| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 76 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | AKAP95 Rabbit Polyclonal Antibody detects endogenous levels of AKAP95 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
AKAP95 also known as AKAP8 is a member of the A-kinase anchor protein family. AKAP95′s primary function involves protein kinase A (PKA) signaling compartmentalization, which is essential for maintaining specific localization within the cell. During mitosis, AKAP95 is recruited to chromosomes, playing a pivotal role in mitotic progression. AKAP95 interacts with the RII regulatory subunit of PKA, facilitating precise signaling necessary for various cellular processes. AKAP95 forms a complex with HA95 and histone deacetylase 3 (HDAC3), which is critical for Histone H3 deacetylation during mitosis, thereby influencing gene expression and chromatin structure. AKAP95′s ability to anchor PKA and other signaling molecules underscores its importance in cellular signaling pathways and highlights its potential as a target for therapeutic interventions in diseases where these pathways are dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, PC12, Jurkat, Mouse heart cells, using AKAP95 Rabbit Polyclonal Antibody (EAB11705) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue, using AKAP95 Rabbit Polyclonal Antibody (EAB11705) at 1:100 dilution.
 Immunofluorescence analysis of HuvEc cell, using AKAP95 Rabbit Polyclonal Antibody (EAB11705) at 1:100 dilution.
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