| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 59 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ATE1 Rabbit Polyclonal Antibody detects endogenous levels of ATE1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ATE1 also known as Arginyltransferase 1 is a crucial enzyme belonging to the R-transferase family, responsible for posttranslational modification of proteins through arginylation, which is essential for subsequent degradation via the ubiquitin-proteasome pathway. This modification is particularly important for proteins containing N-terminal aspartate, glutamate, and possibly cysteine residues, as ATE1 marks them for recognition and degradation, thereby regulating various cellular processes and maintaining protein homeostasis. ATE1 exists as a 518 amino acid monomeric protein that can localize to both cytoplasm and nucleus, highlighting a versatile role in cellular function. Alternative splicing of the ATE1 gene results in two distinct isoforms, which may have different regulatory roles or tissue distributions, further emphasizing the complexity of ATE1 function in cellular biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205 cells, using ATE1 Rabbit Polyclonal Antibody (EAB11709) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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