| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ARF5 Rabbit Polyclonal Antibody detects endogenous levels of ARF5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ARF5(ARF GTPase 5) is a member of the human ADP-ribosylation factor (ARF) gene family. ARF5 plays a crucial role in regulating vesicle trafficking and signal transduction within eukaryotic cells. ARF5 is primarily located in the Golgi apparatus, where ARF5 is essential for maintaining organelle integrity and facilitating the assembly of coat proteins vital for vesicle formation. This localization allows ARF5 to coordinate interactions between spectrin and Golgi membranes, as well as mediate association of actin with the Golgi through localization of Rho family G-proteins, such as Rac and CDC42, along with WASP/Arp2/3 complexes. ARF5 functions critically in cellular processes such as membrane trafficking, which impacts various physiological functions and cellular responses. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116 cells, using ARF5 Rabbit Polyclonal Antibody (EAB14890) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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