| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 41 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Aprataxin Rabbit Polyclonal Antibody detects endogenous levels of Aprataxin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Aprataxin also known as AOA, APTX is a member of the histidine triad (HIT) superfamily and mediates protein-protein interactions with other molecules involved in cellular response to DNA damage. The structure of Aprataxin includes three conserved domains: an N-terminal forkhead-associated (FHA) domain that facilitates these interactions, a HIT domain that shares similarities with Hint, and a C-terminal zinc finger domain that contributes to stability and function. Mutations in the APTX gene, which encodes Aprataxin, can lead to protein destabilization and are associated with a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal head and eye movements. These mutations typically affect the HIT domain or result in truncation before the zinc finger, underscoring Aprataxin′s structural integrity for DNA repair and cellular health. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116 cells, using Aprataxin Rabbit Polyclonal Antibody (EAB14892) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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