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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 96 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Alix Rabbit Polyclonal Antibody detects endogenous levels of Alix protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Alix also known as AIP1, PDCD6IP is a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Alix is primarily located in the cytoplasm, where Alix interacts with several key proteins, including apoptosis-associated proteins such as ALG-2 and PDCD6, as well as endocytosis-regulating proteins like CIN85. Additionally, Alix is involved in recruiting endosomal sorting complexes required for transport (ESCRT) proteins, such as Tsg101 and CHMP4, which are vital for multivesicular body formation and HIV-1 budding regulation. Alix binding with endophilins, enzymes that modify membrane curvature, underscores Alix importance in early and late stages of coated vesicle formation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HT29, HepG2, NIH/3T3 cells, using Alix Rabbit Polyclonal Antibody (EAB11712) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 96 kDa; Observed band size: 85-100 kDa |
