| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 120 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bub1b Rabbit Polyclonal Antibody detects endogenous levels of Bub1b protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bub1b also known as BUB1beta, BUBR1 is a kinase involved in spindle checkpoint function. Bub1b plays a critical role in the mitotic checkpoint, ensuring that chromosomes are properly aligned before cell division proceeds. Bub1b localizes to the kinetochore, where Bub1b interacts with microtubules to monitor their attachment and stability. This localization prevents the premature separation of sister chromatids, safeguarding genomic integrity during cell division. Disruption of Bub1b function can lead to aneuploidy and has been implicated in various cancers, highlighting Bub1b′s importance in maintaining proper cell cycle regulation. Bub1b interacts with other key proteins such as BUB3, which is essential for the spindle assembly checkpoint, further underscoring Bub1b′s role in the complex network of proteins that govern mitosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2 cells, using Bub1b Rabbit Polyclonal Antibody (EAB11719) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 120 kDa; Observed band size: 130 kDa |
