| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 28 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BAP31 Rabbit Polyclonal Antibody detects endogenous levels of BAP31 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BAP31 also kown as CDM, BCAP31 is a member of the B-cell receptor associated protein 31 superfamily. BAP31 plays a significant role in apoptosis regulation. BAP31 forms a protein complex that connects initiator caspases, such as procaspase-8, with anti-apoptotic proteins like Bcl-2 and Bcl-XL, thereby influencing cell survival and death pathways. The cytosolic domain of BAP31 features two identical caspase recognition sites, which are specifically cleaved by initiator caspases during apoptosis, resulting in the generation of a p20 fragment that remains membrane-integrated and acts as a potent inducer of cell death when ectopically expressed. This cleavage is vital for triggering cytoplasmic apoptotic events, including membrane fragmentation, and facilitates the interaction between mitochondria and the endoplasmic reticulum during Fas-mediated apoptosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, HepG2, 22RV1 cells, using BAP31 Rabbit Polyclonal Antibody (EAB11724) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
